Quantitation of Total Protein using OPA
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چکیده
Total protein content is a measurement common to many applications in basic science and clinical research. Several different fluorescent techniques are available that eliminate many of the problems associated with the traditional absorbance-based colorimetric methods to measure total protein content. Here we describe the use of the compound o-phthaldialdehyde (OPA) to quantitate total protein using the BioTek FL600 fluorescence microplate reader.
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